تاثیر تمرین استقامتی بر بیان ژن hp-1β بطن چپ رت‌های نر نژاد ویستار

Background: Epigenetic changes is one of the most important outcomes of Physical activity results, and Heterochromatin Protein 1&beta; is one of main factors of this processes. The aim of this study was to investigate the effect of endurance training on left ventricle hp-1&beta; gene expression in Wistar male rats. Methods: 20 rats (241&plusmn;7.3 g) 8 weeks of age randomly assigned to control (n=10) and experimental (n=10) groups. The experimental group performed a 14-week treadmill running program (6 days per week, with 30 m/min speed), then heart dimensions and weight were determined using m-mode and weighing, respectively. They were anesthetized and sacrificed 48 hours after. The heart was extracted and then Real time-PCR method was used to determine hp-1&beta; gene expression level of in the left ventricle. Finally the data obtained were evaluated using t-test. Results: weighted indexes showed that the weight of isolated left ventricle and heart in experimental group more than control group, and also left ventricle of experimental group was bigger than control group, as the left ventricle-to-body weight ratio of experimental group (2.3&plusmn;0.18) was significantly more than control group (2.049&plusmn;.12). Also the left ventricle-to-BSA ratio of experimental group (0.168&plusmn;0.008) was significantly more than control group (0.153&plusmn;0.006), these finding was coincided with significantly (p<0.05) increase of hp-1 gene expression (49 fold) in left ventricle of experimental group. Conclusion: The results of this study showed that endurance activity induce increase in heart internal dimension and also weight of it especially in left ventricle that this structural changes was coincided with hp-1beta gene expression change. This means each tissue remodeling was accompanied with gene change.